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Protein Expression and Purification Services
 
 
At Bhat Bio-Tech, protein expression specialists work closely with clients to clone and express full-length recombinant proteins from full-length cDNAs, EST sequence information, and genomic sequences, providing service and expertise at all stages of protein expression and purification. The purified proteins can be used in number of down stream applications including monoclonal and polyclonal antibody development, immunoassay development etc. Protein expression and purification service package include sub-cloning into appropriate expression vector, sequence confirmation by gene sequencing, plasmid maxi preparation, optimization of protein expression, expression confirmation by SDS-PAGE and Western blotting, and purification of expressed protein to near homogeneity (>90% pure).
 
Features:
Protein expression in-Bacteria, Yeast, Baculovirus and Mammalian cells are available
Protein purification to near homogeneity (purity >90%)
Expression systems with 6X His tag, GST tag, GFP tag and without any tag are available
Large scale production of recombinant proteins upon request
Purified proteins with endotoxin level < 0.01EU/μg of protein is available on request
 
Deliverables:
All clones and sub-clones generated
Sequence data of the final clone
Purified recombinant protein
Project report
Quality assurance certificate
 

  Cat. No.

  Service

  BBCS-20110

  Protein expression in Bacteria

  BBCS-20111

  Protein expression in Yeast

  BBCS-20112

  Protein expression in Baculovirus

  BBCS-20113

  Protein expression in Mammalian cells

 
 
 
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