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Molecular Biology Services |
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Products >> Contract Research Services >> Molecular Biology Services |
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| PCR Cloning and Sub-cloning service |
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Gene cloning is the act of making copies of a single gene. Amplified genes are useful in many areas of
research ranging from genetic fingerprinting to large scale protein production as well as for medical
applications such as gene therapy. Bhat Bio-Tech offers PCR cloning and sub-cloning services to meet
the needs of the scientists. Our sub-cloning service is highly flexible to any specific project requirements.
The starting materials can be bacterial culture, plasmid DNA or PCR product. |
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| Sample requirements: |
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The complete sequence of the template including the target gene and its vector |
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If the starting material is DNA, 2 μg template DNA |
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Sufficient amount of culture if starting material is bacterial culture |
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| Deliverables: |
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5 μg of destination vector containing the gene |
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Sequence chromatograms covering your gene |
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Construct map for the plasmid |
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Project report |
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Quality assurance certificate |
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| cDNA synthesis and cDNA cloning service |
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A cDNA is a DNA copy synthesized from mRNA. The central part of any cDNA cloning procedure is synthesis
of the cDNA from an mRNA template using reverse transcriptase enzyme. Cloning and sequencing of cDNA is
useful to understand various aspects of the gene as well as to express the genes in heterologous systems. |
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| Features: |
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Cloning in any commercially available cloning or expression vector |
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Cloning and sequencing irrespective of the size of the gene |
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Rapid turnaround time |
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| Deliverables: |
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Full length cDNA in a vector of choice |
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Sequence data |
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Project report |
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Quality assurance certificate |
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Cat. No. |
Service |
BBCS-20101 |
PCR cloning and sub-cloning service |
BBCS-20102 |
cDNA synthesis & cDNA cloning service |
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| DNA sequencing Service |
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Bhat Bio-Tech offers DNA sequencing service using latest and the highest quality high throughput
technologies for its sequencing. |
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| Sample requirements: |
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DNA sample: Plasmid DNA - Purified DNA or bacterial culture to isolate plasmid and to sequence
PCR product - Purified PCR product or PCR reaction for clean up and to sequence
Bacterial culture or template - To PCR amplify, clean up and sequence |
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Primers 10 μM |
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| Deliverables: |
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Sequence data |
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Cat. No. |
Service |
BBCS-20103 |
DNA sequencincing up to 600 bases |
BBCS-20104 |
Purifucation of PCR product and sequencing |
BBCS-20105 |
PCR amplification,Clean up and sequencing |
BBCS-20106 |
Purification of plasmid and sequencing |
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| Large scale plasmid DNA preparation service (endotoxin free) |
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Plasmid DNA preparation service at Bhat Bio-Tech covers individual constructs for research labs as well as large scale manufacturing for biotech and pharmaceutical companies. The high quality plasmids will help you achieve highly efficient cell transfection, DNA vaccination, antibody production, and other preclinical studies. |
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| Features: |
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Flexible scale |
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Stringent quality control |
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Endotoxin level < 0.1 EU/μg DNA (Optional) |
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Rapid turnaround time |
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| Deliverables: |
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Purified plasmid DNA |
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Project report |
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Quality assurance certificate |
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Cat. No. |
Service |
BBCS-20107 |
Large scale plasmid DNA preparation service |
BBCS-20108 |
Large scale plasmid DNA preparation service (Endotoxin free) |
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| Genetically Modified organism (GMO) detection service |
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A genetically modified organism (GMO) is an organism whose genetic material has been altered using genetic engineering techniques giving the organism modified or novel genes. Testing on GMOs in food and feed is routinely done by molecular techniques. We offer multiplex PCR system to screen samples for different potential GMOs. To avoid any kind of false positive or false negative testing outcome, internal controls for every step is performed. |
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| Deliverables: |
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Gel image of PCR result |
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Final report of GMO detection data |
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Cat. No. |
Service |
BBCS-20109 |
GMO detection service |
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