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Products >> Contract Research Services >> Antibody Services |
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| Polyclonal & Monoclonal Antibody Development Service |
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Bhat Bio-Tech provides customized hybridoma development service to meet the customer needs. We can develop monoclonal antibodies to a wide range of antigens including peptides. All the animal experiments are carried out in our state-of-the art facility approved by CPCSEA, Govt. of India.
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| The monoclonal antibody development service is divided into four phases |
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Phase II: Fusion, plating and screening |
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Phase III: Subcloning by limiting dilution, expansion and cryopreservation of 3 selected clones |
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Phase IV: Amplification- production of 50 ml of culture supernatant containing monoclonal antibody. |
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| Sample requirements: |
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5 mg of pure protein or 25-30 mg of peptide |
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| Deliverables: |
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Phase I: ELISA data for antibody titration |
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Phase II: ELISA data for screening of hybridoma |
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Culture supernatant from 5 of the best hybridoma containing wells |
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Phase III: ELISA data for screening antibody secreting subclones |
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Culture supernatant from 3 of monoclines |
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Isotyping results of 3 of the monoclonal antibody |
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Phase IV: 50 ml of culture supernatant containing monoclonal antibody |
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Frozen hybridoma cells |
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Final project report |
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| Additional Hybridoma related services: |
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Additional subcloning, expansion and screening of parental clones |
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Cryopreservation of up to 20 clones per month |
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Monoclonal antibody production in ascites of Balb/c mice |
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Monoclonal antibody production in cell culture flask-
50 to 100 ml culture supernatant |
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Isotyping of monoclonal antibodies |
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Cat. No. |
Service |
BBCS-20114 |
Monoclonal antibody development service |
BBCS-20115 |
Monoclonal antibody production in ascites |
BBCS-20116 |
Monoclonal antibody production in cell culture flask |
BBCS-20117 |
Isotyping of monoclonal antibody |
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| Polyclonal Antibody Production Service in Rabbit |
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At Bhat Bio-Tech, we develop custom polyclonal antibodies of highest quality with fast and reliable delivery
times. The antibody screening will be done by direct ELISA and Western blotting |
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| Sample requirements: |
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5 mg of immunogen |
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| Deliverables: |
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2 ml of pre immunized rabbit serum |
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25 to 30 ml of immunized rabbit serum |
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Project report |
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Cat. No. |
Service |
BBCS-20118 |
Polyclonal antibody production service in Rabbit |
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| Antibody Purification Services |
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Bhat Bio-Tech provides a wide range of options for the purification of monoclonal and polyclonal antibodies.
These include relatively common methods such as protein A and protein G affinity as well as protein L and
antigen affinity chromatography. |
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| Sample requirements: |
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Serum sample |
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If antigen affinity chromatography is required 5 mg of
antigen to prepare purification column |
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| Deliverables: |
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Purified antibody |
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Project report |
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Cat. No. |
Service |
BBCS-20119 |
Purification of antibody by Protein A or protein
G affinity chromatography |
BBCS-20120 |
Purification of antibody by Protein L affinity chromatography |
BBCS-20121 |
Purification of antibody by antigen affinity chromatography |
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| Antibody Labeling Services |
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Bhat Bio-Tech offers a wide range of antibody labeling services to meet the requirements of the researchers.
Antibody conjugated to enzymes, biotin or FITC are widely used in a variety of immunotechniques as a
detection reagent. We provide the following conjugation services |
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Conjugation to HRP |
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Conjugation to ALP |
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Conjugation to Biotin |
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Conjugation to FITC |
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Cat. No. |
Service |
BBCS-20122 |
Antibody- HRP conjugation |
BBCS-20123 |
Antibody- ALP conjugation |
BBCS-20124 |
Antibody- Biotin conjugation |
BBCS-20125 |
Antibody- FITC conjugation |
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| Peptide-Carrier conjugation Service |
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| We offer conjugation of synthetic peptide to KLH, BSA or Ovalbumin depending on customer needs. |
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| Sample requirements: |
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Synthetic peptide or amino acid sequence of the peptide |
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| Deliverables: |
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Peptide carrier conjugate |
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Project report |
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Cat. No. |
Service |
BBCS-20126 |
Peptide-KLH conjugation |
BBCS-20127 |
Peptide-BSA conjugation |
BBCS-20128 |
Peptide-Ova conjugation |
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| Colloidal Gold Labeling Service |
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At Bhat Bio-Tech we have a strong expertise in rapid diagnostic kit development using colloidal gold conjugate.
We offer conjugation of colloidal gold to a variety of proteins. We can conjugate proteins to colloidal gold of
varying size such as 10nm, 15nm, 20nm, 40nm and 60nm depending on the customer requirements. |
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| Features: |
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No aggregation |
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OD 540 is 10 or above |
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Uniform size |
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Wide range of particle size |
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Cat. No. |
Service |
BBCS-20129 |
Colloidal gold labeling service |
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